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ContentsAndarius's Blood Angels army attempt 2000ptsDark Water Rising by Mariana HaleOmicron Provisional Galaxy of Clan Blood Spirit. Chromatin immunoprecipitation DNA-protein cross-linking was done for 30 minutes at room temperature by adding formaldehyde at a final concentration of 1% directly to the culture medium. Cell 2002;108:165-170.OpenUrlCrossRefMedlineWeb of Science Rego EM, Pandolfi PP. To show binding of PML-RAR to the endogenous RAR (B) and ID1 (C) genes, ChIP assays were performed in PML-RARpositive NB4 cells and in PML-RARnegative U937 cells. RAR target genes are not restricted to genes that contain a consensus retinoic acid receptorbinding site in their promoter. Incubation of a radioactively labeled DNA probe containing the CCAAT box with cellular protein extracts resulted in a clear shifted complex (Figure 4A lane 1).
Physical interaction between retinoic acid receptor and Sp1: mechanism for induction of urokinase by retinoic acid. Curr Opin Hematol 2001;8:206-211.OpenUrlCrossRefMedlineWeb of Science Pandolfi PP. Crit Rev Eukaryot Gene Expr 2004;14:171-181.OpenUrlCrossRefMedline Friedman AD. Mean values from 3 independent experiments plus or minus SD are shown. Functional NF-Y is necessary for transactivation of the ID1 promoter by PML-RAR Sp1 and NF-Y are ubiquitously expressed transcription factors. performed research and analyzed data; B.L., T.W., and B.A.R. The ID1 upstream promoter is transactivated by PML-RAR but not by RAR/RXR To identify the regulatory DNA sequences through which the induction of ID1 by retinoic acid was mediated, we analyzed the 5 upstream promoter sequence in transactivation assays. To investigate this further, we used a FLAG-tagged PML-RAR expression construct, allowing us to unequivocally identify binding by the PML-RAR fusion protein.
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